Search results for " Sensitivity and Specificity"

showing 10 items of 39 documents

Effect of reducing agents on the acidification capacity and the proton motive force of Lactococcus lactis ssp. cremoris resting cells.

2002

International audience; Reducing agents are potential inhibitors of the microbial growth. We have shown recently that dithiothreitol (DTT), NaBH(4) and H(2) can modify the proton motive force of resting cells of Escherichia coli by increasing the membrane protons permeability [Eur. J. Biochem. 262 (1999) 595]. In the present work, the effect of reducing agents on the resting cells of Lactococcus lactis ssp. cremoris, a species widely employed in dairy processes was investigated. DTT did not affect the acidification nor the DeltapH, in contrast to the effect previously reported on E. coli. The DeltaPsi was slightly increased (30 mV) at low pH (pH 4) in the presence of 31 mM DTT or 2.6 mM NaB…

MESH : Cell LineMESH: Hydrogen-Ion ConcentrationMESH : DithioniteBorohydridesMESH : DithiothreitolBacterial growthmedicine.disease_causeMESH: Proton-Motive ForceDithiothreitolSodium dithionitechemistry.chemical_compoundMESH : Proton-Motive ForceElectrochemistry[INFO.INFO-BT]Computer Science [cs]/Biotechnology0303 health sciencesMESH : Interphasebiologyfood and beveragesProton-Motive ForceGeneral MedicineHydrogen-Ion ConcentrationMESH: BorohydridesLactococcus lactisMembraneBiochemistryReducing AgentsMESH : Sensitivity and SpecificityMESH : Reducing Agents[ INFO.INFO-BT ] Computer Science [cs]/BiotechnologyReducing agentMESH: Reducing AgentsBiophysics[SDV.BC]Life Sciences [q-bio]/Cellular BiologySensitivity and SpecificityCell LineMESH: Interphase03 medical and health sciencesSpecies SpecificityMESH : Hydrogen-Ion ConcentrationMESH: DithionitemedicineMESH : Species SpecificityMESH: Species SpecificityLactic AcidPhysical and Theoretical ChemistryEscherichia coli[SDV.BC] Life Sciences [q-bio]/Cellular BiologyInterphase030304 developmental biology[ SDV.BC ] Life Sciences [q-bio]/Cellular Biology030306 microbiologyChemiosmosisLactococcus lactisDithionitebiology.organism_classificationMESH: Sensitivity and SpecificityMESH: Cell LineDithiothreitol[INFO.INFO-BT] Computer Science [cs]/BiotechnologychemistryMESH: Lactococcus lactisMESH : BorohydridesMESH : Lactic AcidBiophysicsMESH: Lactic AcidMESH : Lactococcus lactisMESH: Dithiothreitol
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Perfusion Index and Pulse Oximetry Screening for Congenital Heart Defects

2017

Objective To evaluate the efficacy of combined pulse oximetry (POX) and perfusion index (PI) neonatal screening for severe congenital heart defects (sCHD) and assess different impacts of screening in tertiary and nontertiary hospitals. Study design A multicenter, prospective study in 10 tertiary and 6 nontertiary maternity hospitals. A total of 42 169 asymptomatic newborns from among 50 244 neonates were screened; exclusion criteria were antenatal sCHD diagnosis, postnatal clinically suspected sCHD, and neonatal intensive care unit admission. Eligible infants underwent pre- and postductal POX and PI screening after routine discharge examination. Targeted sCHD were anatomically defined. Posi…

MalePediatricsNeonatal intensive care unit030204 cardiovascular system & hematologyPediatricsSeverity of Illness IndexHypoplastic left heart syndromeCohort StudiesTertiary Care CentersCongenital0302 clinical medicineNeonatalOximetryProspective StudiesProspective cohort studyHeart Defectsmedicine.diagnostic_testIncidenceIncidence (epidemiology)Perinatology and Child Healthcongenital heart defectsHospitalspulse oximetrycongenital heart defects; neonatal screening; perfusion index; pulse oximetry; Blood Gas Analysis; Cohort Studies; Heart Defects Congenital; Hospitals Maternity; Humans; Incidence; Infant Newborn; Intensive Care Units Neonatal; Italy; Male; Neonatal Screening; Oximetry; Oxygen Consumption; Prospective Studies; Risk Assessment; Sensitivity and Specificity; Severity of Illness Index; Tertiary Care Centers; Pediatrics Perinatology and Child HealthIntensive Care UnitsItalymedicine.symptomCohort studyHeart Defects Congenitalmedicine.medical_specialtyMaternityHospitals MaternityRisk AssessmentSensitivity and SpecificityAsymptomatic03 medical and health sciencesNeonatal ScreeningOxygen ConsumptionIntensive Care Units Neonatal030225 pediatricsSeverity of illnessmedicineHumansperfusion indexbusiness.industryInfant NewbornInfantNewbornmedicine.diseasePulse oximetryPediatrics Perinatology and Child HealthBlood Gas AnalysisbusinessThe Journal of Pediatrics
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Implementation of Sample Pooling Procedure Using a Rapid SARS-CoV-2 Diagnostic Real-Time PCR Test Performed Prior to Hospital Admission of People wit…

2021

Reliability, accuracy, and timeliness of diagnostic testing for SARS-CoV-2 infection have allowed adequate public health management of the disease, thus notably helping the timely mapping of viral spread within the community. Furthermore, the most vulnerable populations, such as people with intellectual disability and dementia, represent a high-risk group across multiple dimensions, including a higher prevalence of pre-existing conditions, lower health maintenance, and a propensity for rapid community spread. This led to an urgent need for reliable in-house rapid testing to be performed prior to hospital admission. In the present study, we describe a pooling procedure in which oropharyngeal…

medicine.medical_specialtyHealth Toxicology and MutagenesisPoolingRT-PCRsample poolingSample (statistics)DiseaseReal-Time Polymerase Chain ReactionSensitivity and SpecificityIntellectual DisabilityIntellectual disabilitymedicineHumansDementiaHospital admission RT-PCR Sample pooling SARS-CoV-2 Sensitivity Hospitals Humans Real-Time Polymerase Chain Reaction Reproducibility of Results SARS-CoV-2 Sensitivity and Specificity COVID-19 Intellectual DisabilitySARS-CoV-2business.industryBrief ReportPublic healthRPublic Health Environmental and Occupational HealthCOVID-19Reproducibility of Resultssensitivitymedicine.diseaseHospitalsTest (assessment)hospital admissionEmergency medicineMedicineSample collectionbusinessInternational Journal of Environmental Research and Public Health
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Performance of existing definitions and tests for the diagnosis of invasive aspergillosis in critically ill, adult patients: A systematic review with…

2020

Contains fulltext : 229471.pdf (Publisher’s version ) (Closed access) OBJECTIVES: To summarize the available evidence on the diagnostic performance for invasive aspergillosis (IA) in non-hematological, non-solid organ transplantation critically ill patients of the following: (i) existing definitions of IA (developed either for classical immunocompromised populations or for non-immunocompromised critically ill patients); (ii) laboratory tests; (iii) radiology tests. METHODS: A systematic review was performed by evaluating studies assessing the diagnostic performance for IA of a definition/s and/or laboratory/radiology test/s vs. a reference standard (histology) or a reference definition. RES…

0301 basic medicineMicrobiology (medical)Adultmedicine.medical_specialtyCritical Illness030106 microbiologyAspergillosisSensitivity and SpecificityOrgan transplantationMannans03 medical and health sciencesGalactomannanchemistry.chemical_compound0302 clinical medicineDiagnosisMedicineHumansAspergillosis030212 general & internal medicineIntensive care medicineInvasive Pulmonary AspergillosisAdult patientsmedicine.diagnostic_testbusiness.industryCritically illIABiomarkerInvasive pulmonary aspergillosismedicine.diseaseAspergillulnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]Infectious DiseasesBronchoalveolar lavageAspergilluschemistryIPAAspergillus; Biomarker; Diagnosis; IA; IPA; Invasive aspergillosis; Adult; Bronchoalveolar Lavage Fluid; Critical Illness; Humans; Mannans; Sensitivity and Specificity; Aspergillosis; Invasive Pulmonary AspergillosisBiomarker (medicine)Aspergillus; Biomarker; Diagnosis; IA; Invasive aspergillosis; IPA; Adult; Bronchoalveolar Lavage Fluid; Critical Illness; Humans; Mannans; Sensitivity and Specificity; Aspergillosis; Invasive Pulmonary AspergillosisInvasive aspergillosisbusinessBronchoalveolar Lavage FluidDiagnosi
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Normal Reference Ranges for Echocardiography: Rationale, study design, and methodology (NORRE Study)

2013

International audience; BACKGROUND: Availability of normative reference values for cardiac chamber dimensions, volumes, mass, and function is a prerequisite for the accurate application of echocardiography for both clinical and research purposes. However, due to the lack of consistency in current echocardiographic 'reference values', their use for clinical decision-making remains questionable. AIMS: The aim of the 'Normal Reference Ranges for Echocardiography Study (NORRE Study)' is to obtain a set of 'normal values' for cardiac chamber geometry and function in a large cohort of healthy Caucasian individuals aged over a wide range of ages (25-75 years) using both conventional and advanced e…

MaleRadiology Nuclear Medicine and ImagingMESH: Echocardiography DopplerLeftSex Factor030204 cardiovascular system & hematologyDoppler echocardiographyDoppler imagingMESH: Stroke VolumeMESH: Ventricular Function LeftVentricular Function Left030218 nuclear medicine & medical imagingHeart VentricleCohort Studies0302 clinical medicine[INFO.INFO-TS]Computer Science [cs]/Signal and Image ProcessingNuclear Medicine and ImagingVentricular FunctionAge FactorProspective StudiesProspective cohort studyMESH: Cohort StudiesMESH: Aged2D and 3D echocardiographyMESH: Middle Agedmedicine.diagnostic_testAnthropometryAge FactorsDopplerGeneral MedicinePulsedReference Standardsreference valuesMiddle AgedCardiac mechanicEchocardiography Doppler3. Good healthEuropeMESH: Echocardiography Doppler PulsedCardiac mechanicsEchocardiographyMESH: Reference Standards[SDV.IB]Life Sciences [q-bio]/BioengineeringFemaleRadiologyCardiology and Cardiovascular Medicine[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processingCohort study2D and 3D echocardiography; Cardiac mechanics; Chamber size and function; M-mode; reference values; Adult; Age Factors; Aged; Anthropometry; Cohort Studies; Echocardiography Doppler; Echocardiography Doppler Pulsed; Europe; Female; Heart Ventricles; Humans; Male; Middle Aged; Prospective Studies; Reference Standards; Sensitivity and Specificity; Sex Factors; Stroke Volume; Ventricular Function Left; Cardiology and Cardiovascular Medicine; Radiology Nuclear Medicine and ImagingHumanAdultmedicine.medical_specialtyHeart VentriclesM-modeChamber size and functionSensitivity and Specificity03 medical and health sciencesSex FactorsMESH: Sex FactorsMESH: Anthropometry[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemmedicineHumansMedical physicsAgedMESH: Age FactorsEchocardiography Doppler PulsedReproducibilityMESH: Humansbusiness.industryreference valueMESH: AdultStroke VolumeMESH: MaleMESH: Prospective StudiesMESH: Sensitivity and SpecificitySurgeryClinical trialProspective StudieSample size determinationReference StandardObservational studyMESH: EuropeMESH: Heart VentriclesCohort StudiebusinessMESH: Female
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Validity of needle core biopsy in the histological characterisation of mammary lesions

2006

Summary Over the last few years, there has been an enormous increase in the use of needle core biopsy (CB) for the histopathological characterisation of suspect lesions of the breast. The aim of this study was to verify the diagnostic reliability of CB by comparing the histological results obtained with the use of this technique with those obtained from the whole of the surgically resected specimen. We studied 198 out of 426 patients with clinically and/or radiologically suspect breast lesions. We found correspondence between the histological examination of the whole of the excised specimen and that of the CB in 94.9% of the cases of infiltrating carcinoma and in 71.4% of those involving du…

Pathologymedicine.medical_specialtyReproducibility of ResultBreast NeoplasmsSensitivity and SpecificityBreast cancerNeedle core biopsyRetrospective StudiemedicineCarcinomaHumansbreast carcinomahumanintermethod comparisonarticle; breast carcinoma; breast disease; carcinoma in situ; diagnostic accuracy; diagnostic value; histopathology; human; human tissue; intermethod comparison; intraductal carcinoma; major clinical study; needle biopsy; prediction; priority journal; reliability; sensitivity and specificity; validation process Biopsy Needle; Breast Neoplasms; Carcinoma Ductal Breast; Carcinoma Intraductal Noninfiltrating; Female; Humans; Reproducibility of Results; Retrospective Studies; Sensitivity and Specificity [EMTREE medical terms]Retrospective Studiesintraductal carcinomaHistological examinationreliabilitybusiness.industryBiopsy Needlevalidation process MeSH: Biopsy NeedleCarcinoma Ductal Breastbreast diseaseReproducibility of Resultscarcinoma in situneedle biopsypredictionGeneral Medicinemedicine.diseasemajor clinical studyPredictive valuehuman tissueCarcinoma Intraductal Noninfiltratingpriority journalhistopathologyEMTREE medical terms: articlediagnostic accuracyFemaleSurgerydiagnostic valueRadiologybusinessCore biopsyBreast NeoplasmThe Breast
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Introduction to coronary imaging with 64-slice computed tomography

2005

The aim of this article is to illustrate the main technical improvements in the last generation of 64-row CT scanners and the possible applications in coronary angiography. In particular, we describe the new physical components (X-ray tube-detectors system) and the general scan and reconstruction parameters. We then define the scan protocols for coronary angiography with the new generation of 64-row CT scanners to enable radiologists to perform a CT study on the basis of the diagnostic possibilities.

Time FactorsImaging Sensitivity and Specificity Stents Time Factors TomographyContrast MediaSensitivity and Specificity64-row CTElectrocardiographyHeart RateSpiral Computed TomographyImage Processing Computer-AssistedHumanstechnical improvementsCoronary Artery BypassPhantoms ImagingPatient SelectionAlgorithms Artifacts Contrast Media Coronary Angiography/instrumentation/*methods Coronary Artery Bypass Coronary Stenosis/*radiography Electrocardiography Heart Rate Humans Image Processing; Computer-Assisted Patient Selection Phantoms; Imaging Sensitivity and Specificity Stents Time Factors Tomography; Spiral Computed Tomography; X-Ray Computed/instrumentation/*methodsCoronary StenosisComputer-Assisted Patient Selection PhantomsX-Ray Computed/instrumentation/*methodstechnical improvements; 64-row CT; coronary angiographyComputer-Assisted Patient Selection PhantomStentsAlgorithms Artifacts Contrast Media Coronary Angiography/instrumentation/*methods Coronary Artery Bypass Coronary Stenosis/*radiography Electrocardiography Heart Rate Humans Image Processingcoronary angiographyArtifactsTomography X-Ray ComputedTomography Spiral ComputedAlgorithms
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Diagnostic accuracy of computed tomographic colonography for the detection of advanced neoplasia in individuals at increased risk of colorectal cance…

2009

CONTEXT: Computed tomographic (CT) colonography has been recognized as an alternative for colorectal cancer (CRC) screening in average-risk individuals, but less information is available on its performance in individuals at increased risk of CRC. OBJECTIVE: To assess the accuracy of CT colonography in detecting advanced colorectal neoplasia in asymptomatic individuals at increased risk of CRC using unblinded colonoscopy as the reference standard. DESIGN, SETTING, AND PARTICIPANTS: This was a multicenter, cross-sectional study. Individuals at increased risk of CRC due to either family history of advanced neoplasia in first-degree relatives, personal history of colorectal adenomas, or positiv…

Maleoccult blood testColorectal cancerColonoscopycancer riskprediction and forecastingGastroenterologyRisk Factorscancer diagnosisPositive predicative valueadvanced cancerMedicineFamily historycomparative studycolorectal adenomafamily historyeducation.field_of_studymedicine.diagnostic_testarticleclinical trialGeneral MedicineColonoscopyMiddle Agedpriority journalrisk factorPredictive value of testsdiagnostic accuracyFemalediagnostic valueColorectal NeoplasmsColonography Computed TomographicradiographyComputed TomographicAdultmedicine.medical_specialtySettore MED/12 - GASTROENTEROLOGIAPopulationcolorectal cancerSensitivity and SpecificityPredictive Value of TestsInternal medicineadult; advanced cancer; aged; article; cancer classification; cancer diagnosis; cancer risk; clinical trial; colonoscopy; colorectal adenoma; colorectal cancer; computed tomographic colonography; controlled clinical trial; controlled study; cross-sectional study; diagnostic accuracy; diagnostic value; family history; female; human; major clinical study; male; multicenter study; occult blood test; predictive validity; priority journal; colorectal tumor; comparative study; middle aged; prediction and forecasting; radiography; risk factor; sensitivity and specificity; Adult; Aged; Colonography Computed Tomographic; Colonoscopy; Colorectal Neoplasms; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Predictive Value of Tests; Risk Factors; Sensitivity and Specificitycross-sectional studyHumanscontrolled studyhumaneducationAgedcontrolled clinical trialbusiness.industryFecal occult bloodSigmoidoscopyColonographymedicine.diseasecancer classificationcomputed tomographic colonographymajor clinical studypredictive validitymulticenter studyCross-Sectional StudiesCT colonography colorectal cancer screeningSettore MED/36 - Diagnostica Per Immagini E Radioterapiabusinesscolorectal tumorJAMA
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Convergence of Wnt signaling on the HNF4alpha-driven transcription in controlling liver zonation.

2009

Background & Aims: In each hepatocyte, the specific repertoire of gene expression is influenced by its exact location along the portocentrovenular axis of the hepatic lobule and provides a reason for the liver functions compartmentalization defined "metabolic zonation." So far, few molecular players controlling genetic programs of periportal (PP) and perivenular (PV) hepatocytes have been identified; the elucidation of zonation mechanisms remains a challenge for experimental hepatology. Recently, a key role in induction and maintenance of the hepatocyte heterogeneity has been ascribed to Wnt/β-catenin pathway. We sought to clarify how this wide-ranging stimulus integrates with hepatocyte s…

Beta-cateninWnt ProteinCellular differentiationBlotting WesternLiver Stem CellFluorescent Antibody TechniqueMice TransgenicBiologyTransfectionSensitivity and SpecificityAnimals; Blotting Western; Cell Differentiation; Cell Proliferation; Cells Cultured; Fluorescent Antibody Technique; Hepatocyte Nuclear Factor 4; Hepatocytes; Humans; Immunoprecipitation; Mice; Mice Knockout; Mice Transgenic; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Signal Transduction; Transfection; Wnt Proteins; beta Catenin; GastroenterologyMiceliver zonation; wnt signalling; beta catenin; hnf4Gene expressionmedicineAnimalsHumansImmunoprecipitationHepatocyteCells Culturedbeta CateninCell ProliferationMice KnockoutHepatologyAnimalReverse Transcriptase Polymerase Chain ReactionGastroenterologyWnt signaling pathwayCell DifferentiationMolecular biologyWnt Proteinsmedicine.anatomical_structureHepatocyte nuclear factor 4Hepatocyte Nuclear Factor 4Hepatocytebiology.proteinHepatocytesChromatin immunoprecipitationHumanSignal TransductionGastroenterology
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Evaluation of seven immunochromatographic assays for the rapid detection of human rotaviruses in fecal specimens

2007

International audience; Seven commercially available immunochromatographic assays were tested for the rapid detection of group A rotaviruses in fecal samples compared to a enzyme immunoassay (Argene). Detection of rotaviruses in 80 ELISA positive frozen stool samples showed rates superior to 90% for three reagents (Rota Strip (Cypress Diagnostics), 98.8%; Rotascreen (Microgen), 95.0%; VIKIA Rota/Adeno (bioMérieux), 92.5%); from 82.5% to 88.8% for three others (Diarlex with centrifugation (Orion Diagnostica), 88.8%; Combo Rota/Adeno (All Diag), 87.5%; Rota/Adeno Combi Stick (bmd), 82.5%) and only 70.0% for Diarlex with filtration vial (Orion Diagnostica). The evaluation of the specificity, p…

Rotavirustests rapidesMESH : RotavirusdiagnosticMESH: Chromatography[ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/VirologySensitivity and SpecificityFeces[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyHumansComputingMilieux_MISCELLANEOUSMESH : ChromatographysellesImmunoassay[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/VirologyChromatographyimmunochromatographieMESH: HumansMESH : HumansMESH: FecesMESH : FecesMESH: RotavirusMESH : ImmunoassaydétectionMESH: Sensitivity and Specificity[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMESH : Sensitivity and SpecificitygastroenteritisMESH: Immunoassay
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